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KMID : 0043320080310010047
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Archives of Pharmacal Research
2008 Volume.31 No. 1 p.47 ~ p.53
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15,16-Dihydrotanshinone I, a Major Component from Salvia Miltiorrhiza Bunge(Dansham), Inhibits Rabbit Platelet Aggregation by Suppressing Intracellular Calcium Mobilization
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Park Jung-Woo
Chung Hun-Jong
Lee Chang-Soo
Shin Hwa-Sup
Lee Sung-Hun
Yang Min-Kyu
Song Min-Jin
Kwon Suk-Hyung
Yun Yeo-Pyo
Choi Wahn-Soo
Lee Jung-Jin
Ryu Si-Yong
Won Hyung-Sik
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Abstract
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Radix Salviae miltiorrhiza (RSM, ’Dansham’ in Korea, ’Danshen’ in Chinese), the root of Salviae miltiorrhiza Bunge (Labiate) has been used as Chinese fork medicine for the treatment of cardiovascular diseases such as angina pectoris, coronary heart disease, myocardial infarction, and hypertension. In the present study, we evaluated the inhibitory effects of 15,16-Dihydrotanshinone I, one of the major ingredients of Salvia miltiorrhiza Bunge, on platelet aggregation, with elucidation of its mechanisms of action. 15,16-Dihydrotanshinone I concentration-dependently inhibited collagen-induced aggregation of rabbit washed platelets with IC50 of 8.7±5.6 μM, the potency being about seven-fold greater than EGCG, an active Green tea catechin component (IC50 : 56.6±48.7 μM). 15,16-Dihydrotanshinone I significantly inhibited the intracellular calcium mobilization in a concentration-dependent manner. 15,16-dihdydrotanshinone I also significantly suppressed collagen (50 )-induced liberation of Arachidonic acid from Arachidonic acid-incorporated rabbit platelet. In addition, 15,16-Dihydrotanshinone I at 50 μM slightly but significantly inhibited collagen-induced production of thromboxane B2. These results indicate that 15,16-Dihydrotanshinone I exert potent anti-platelet activity via suppression of mobilization and arachidonic acid liberation.
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KEYWORD
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15, 16-Dihydrotanshinone I, Dansham, Salviae, Washed rabbit platelet, Collagen, Calcium
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